A series of flight tests were flown to measure pilot air-to-air visual acquisition performance for pilots employing unalerted visual search. Twenty-four general aviation subject pilots flew a cross-country route while an intercepting aircraft was controlled to produce three intercepts with altitude separation of 500 feet. Pilots received no traffic advisory information to alert them to the possible presence of the intercepting aircraft. Results were analyzed to estimate the instantaneous rate of visual acquisition for a visual target of specified size and contrast. The results were used to calibrate a mathematical model of visual acquisition that can be used to predict pilot performance under a range of conditions.